Effect of Vitreoscilla hemoglobin expression on growth and specific tissue plasminogen activator productivity in recombinant Chinese hamster ovary cells

Journal Article · Thu Dec 01 00:00:00 EST 1994 · Biotechnology and Bioengineering; (United States)

DOI:https://doi.org/10.1002/bit.260441114· OSTI ID:6723195

Pendse, G J; Bailey, J E ^[1]

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1. California Inst. of Tech., Pasadena, CA (United States). Dept. of Chemical Engineering

Previous studies suggest that secretion of cloned proteins synthesized by recombinant Chinese hamster ovary (CHO) cells can be adenosine triphosphate (ATP) limited. Other research indicates that the presence of cloned Vitreoscilla hemoglobin (VHb) enhances ATP production in oxygen-limited Escherichia coli. To evaluate the influence of VHb expression on recombinant CHO cell productivity, the vhb gene has been fused to the mouse mammary tumor virus (MMTV) promoter and cloned in a CHO cell line previously engineered to express human tissue plasminogen activator (tPA). Western blot analysis confirms dexamethasone-inducible VHb expression in all of the clones tested. Batch cultivation experiments with one VHb-expressing clone and the parental CHO-tPA cells show a reduced specific growth rate in the VHb-expressing cells. The VHb-expressing clone exhibits specific tPA production 40 to 100% greater than the parental CHO-tPA culture.

• OSTI ID: 6723195
• Journal Information: Biotechnology and Bioengineering; (United States), Vol. 44:11; ISSN 0006-3592
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHO CELLS
GROWTH
HEMOGLOBIN
GENES
BIOTECHNOLOGY
CLONING
GENE REGULATION
POST-TRANSLATION MODIFICATION
RECOMBINANT DNA
ANIMAL CELLS
CARBOXYLIC ACIDS
DNA
GLOBINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
550200* - Biochemistry