Aquifer microcosms and in situ methods to test the fate and function of pollutant-degrading microorganisms

Technical Report · Mon Jan 01 04:00:00 EST 1990

DOI:https://doi.org/10.2172/6906240· OSTI ID:6906240

Krumme, M L; Dwyer, D ^[1]; Thiem, S M; Tiedje, J M ^[2]; Smith, R L ^[3]

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1. Kernforschungsanlage Juelich GmbH (Germany, F.R.). Inst. fuer Biotechnologie
2. Michigan State Univ., East Lansing, MI (USA)
3. Geological Survey, Reston, VA (USA)

Little information is available on groundwater microorganism ecology, and specifically on the distribution and biochemical diversity of pollution degrading microorganisms in the aquifer. While the introduction of nutrients and electron acceptors may stimulate natural populations to degrade certain pollutants, low levels of pollutants and complex mixtures of pollutants may require the modification of natural populations through selective pressure or by means of genetic engineering. This study was designed to address these issues by examining three populations of substituted aromatic compound-degraders: an indigenous population, an introduced degrader, and a genetically engineered microorganism (GEM) in the environmental conditions of a sand and gravel aquifer. The goals of this study are to gain field experience on the fate and function of pollutant-degrading organisms in the aquifer and to evaluate column microcosms and survival chambers as tools for predicting the fate and function of selected and modified bacterial strains as appropriate aquifer bioremediation agents. 6 figs.

• Research Organization: Michigan State Univ., East Lansing, MI (USA)
• Sponsoring Organization: DOE/ER
• DOE Contract Number: FG02-89ER60848
• OSTI ID: 6906240
• Report Number(s): DOE/ER/60848-1; ON: DE90009956
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540320 -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (1990-)
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ALKYLATED AROMATICS
AQUATIC ECOSYSTEMS
AQUIFERS
AROMATICS
BACTERIA
BIOCHEMISTRY
BIODEGRADATION
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
ECOLOGY
ECOSYSTEMS
FEDERAL REGION I
GENETIC ENGINEERING
GROUND WATER
HYDROCARBONS
HYDROGEN COMPOUNDS
HYDROLOGY
HYDROXY COMPOUNDS
MASSACHUSETTS
MICROCOSMS
MICROORGANISMS
NORTH AMERICA
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXYGEN COMPOUNDS
PHENOLS
PLUMES
POLLUTANTS
POPULATION DYNAMICS
PSEUDOMONAS
REMEDIAL ACTION
SALTS
TOLUENE
USA
WATER