Development of aquifer microcosms and in situ methods to test the fate and function of pollutant-degrading microorganisms
Technical Report
·
OSTI ID:5941878
Our research goals for the past year were to: (1) validate the use of microcosms as predictors of microorganism survival in a polluted aquifer, and (2) develop sensitive methods for detecting the introduced microorganism, Pseudomonas sp. B13 (B13) a 3-chlorobenzoate degrader, from aquifer samples using molecular biology techniques, without the need for culturing. The microcosm and aquifer field studies were carried out at the US Geological Survey's Cape Cod study site at Camp Edwards in Falmouth, Massachusetts. Potential gene probes were developed at the German Institute for Biotechnology (GBF) and at Michigan State University (MSU). The Gene probes were evaluated for use in this project at MSU. The results suggest that microcosms could serve as models for short-term survival, but may not be reliable predictors of microbial fates for longer periods of time under some nutrient and redox conditions. Through the use of radiolabeled probes or internal primer sets we hope to improve the level of detection to near one organism per gram. 9 figs.
- Research Organization:
- Michigan State Univ., East Lansing, MI (USA). Dept. of Crop and Soil Sciences
- Sponsoring Organization:
- DOE; USDOE, Washington, DC (USA)
- DOE Contract Number:
- FG02-89ER60848
- OSTI ID:
- 5941878
- Report Number(s):
- DOE/ER/60848-2; ON: DE91010748
- Country of Publication:
- United States
- Language:
- English
Similar Records
The use of molecular techniques to evaluate the survival of a microorganism that was injected into an aquifer
Behavior of pollutant-degrading microorganisms in aquifers. Predictions for genetically engineered organisms
Evaluation of terrestrial microcosms for detection, fate, and survival analysis of genetically engineered microorganisms and their recombinant genetic material
Technical Report
·
Fri Oct 01 00:00:00 EDT 1993
·
OSTI ID:10103098
Behavior of pollutant-degrading microorganisms in aquifers. Predictions for genetically engineered organisms
Journal Article
·
Wed Jun 01 00:00:00 EDT 1994
· Environmental Science and Technology; (United States)
·
OSTI ID:7161003
Evaluation of terrestrial microcosms for detection, fate, and survival analysis of genetically engineered microorganisms and their recombinant genetic material
Technical Report
·
Tue Jan 31 23:00:00 EST 1989
·
OSTI ID:6409872
Related Subjects
54 ENVIRONMENTAL SCIENCES
540320 -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (1990-)
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AQUATIC ECOSYSTEMS
AQUIFERS
AROMATICS
BACTERIA
BIOCHEMISTRY
BIODEGRADATION
CHEMICAL REACTIONS
CHEMISTRY
CHLORINATED AROMATIC HYDROCARBONS
DECOMPOSITION
DETECTION
DOCUMENT TYPES
ECOLOGY
ECOSYSTEMS
FEDERAL REGION I
GENETIC ENGINEERING
GROUND WATER
HALOGENATED AROMATIC HYDROCARBONS
HYDROGEN COMPOUNDS
MASSACHUSETTS
MICROCOSMS
MICROORGANISMS
NORTH AMERICA
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXYGEN COMPOUNDS
PLUMES
POLLUTANTS
POPULATION DYNAMICS
PROGRESS REPORT
PSEUDOMONAS
USA
WATER
540320 -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (1990-)
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AQUATIC ECOSYSTEMS
AQUIFERS
AROMATICS
BACTERIA
BIOCHEMISTRY
BIODEGRADATION
CHEMICAL REACTIONS
CHEMISTRY
CHLORINATED AROMATIC HYDROCARBONS
DECOMPOSITION
DETECTION
DOCUMENT TYPES
ECOLOGY
ECOSYSTEMS
FEDERAL REGION I
GENETIC ENGINEERING
GROUND WATER
HALOGENATED AROMATIC HYDROCARBONS
HYDROGEN COMPOUNDS
MASSACHUSETTS
MICROCOSMS
MICROORGANISMS
NORTH AMERICA
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXYGEN COMPOUNDS
PLUMES
POLLUTANTS
POPULATION DYNAMICS
PROGRESS REPORT
PSEUDOMONAS
USA
WATER