Amine N-methyltransferase studies in rat, guinea pig, hamster, and rabbit with special emphasis on nicotine N-methylation in vivo
Investigation of the biological N-methylation of amines has been conducted. Tryptamine and pyridine N-methylation reactions have been evaluated in vitro using rabbit lung and liver homogenates. The N-methylated derivatives of tryptamine and pyridine have been synthesized and used in the development of analytical systems, based upon high performance liquid chromatography. These analytical systems have allowed the unambiguous determination of the identity of the N-methylated derivatives of these amino compounds which are formed in tissue homogenates that have been incubated with S-adenosyl-L-(/sup 3/H-methyl)-methionine, thereby also establishing SAM as the co-factor in these enzymatic reactions. The in vivo metabolism of radiolabeled nicotine enantiomers to oxidative and N-methylated nicotine compounds has been studied. Analyses revealed that significant differences exist in the metabolism of radiolabeled nicotine enantiomers among the different animals species.
- Research Organization:
- Kentucky Univ., Lexington (USA)
- OSTI ID:
- 6905558
- Country of Publication:
- United States
- Language:
- English
Similar Records
Discovery of Bisubstrate Inhibitors of Nicotinamide N-Methyltransferase (NNMT)
Biotransformation of primary nicotine metabolites. I. In vivo metabolism of R-(+)-( UC-NCH3)N-methylnicotinium ion in the guinea pig
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALKALOIDS
AMINES
AMINO ACIDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZINES
AZOLES
BIOLOGICAL PATHWAYS
BODY
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DIGESTIVE SYSTEM
DRUGS
ENZYME ACTIVITY
ENZYMES
GLANDS
GUINEA PIGS
HAMSTERS
HETEROCYCLIC COMPOUNDS
HOMOGENATES
IN VIVO
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
LIQUID COLUMN CHROMATOGRAPHY
LIVER
LUNGS
MAMMALS
METABOLISM
METHIONINE
METHYL TRANSFERASES
METHYLATION
NICOTINE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PARASYMPATHOLYTICS
PARASYMPATHOMIMETICS
PYRIDINES
PYRROLES
PYRROLIDINES
RABBITS
RATS
RESPIRATORY SYSTEM
RODENTS
SEPARATION PROCESSES
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES