Biotransformation of primary nicotine metabolites. I. In vivo metabolism of R-(+)-( UC-NCH3)N-methylnicotinium ion in the guinea pig
The in vivo biotransformation and tissue distribution of the methylated nicotine metabolite R-(+)-( UC-NCH3)N-methylnicotinium acetate was studied in the guinea pig. The detection and quantification of 24-hr urinary metabolites after ip injection was determined by cation-exchange HPLC interfaced to a radiochemical flowthrough detector. The urinary metabolite profile consisted of five peaks. One eluted close to the void, and three coeluted with authentic standards of N-methylcotininium ion, N-methylnornicotinium ion, and N-methylnicotinium ion. A fifth, and as yet unidentified, metabolite was also detected. Tissue distribution of UC label after 24 hr was highest in the adrenal gland and epididymis followed by the gallbladder, bladder, kidney, spleen, and heart. No significant amounts of UC were found in the brain. The results indicate that N-methylcotininium ion and N-methylnornicotinium ion are both formed subsequent to the formation of N-methylnicotinium ion in the metabolism of R-(+)-nicotine in the guinea pig.
- Research Organization:
- Univ. of Kentucky, Lexington
- OSTI ID:
- 6044454
- Journal Information:
- Drug Metab. Dispos.; (United States), Journal Name: Drug Metab. Dispos.; (United States) Vol. 5; ISSN DMDSA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ACETATES
ADRENAL GLANDS
ALKALOIDS
AMINES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZINES
AZOLES
BILIARY TRACT
BLADDER
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACID SALTS
CARDIOVASCULAR SYSTEM
CHEMICAL REACTIONS
CHROMATOGRAPHY
DIGESTIVE SYSTEM
DISTRIBUTION
DRUGS
ENDOCRINE GLANDS
GLANDS
GUINEA PIGS
HEART
HETEROCYCLIC COMPOUNDS
KIDNEYS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
METABOLITES
METHYLATION
NICOTINE
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PARASYMPATHOLYTICS
PARASYMPATHOMIMETICS
PYRIDINES
PYRROLES
PYRROLIDINES
RODENTS
SEPARATION PROCESSES
SPLEEN
TISSUE DISTRIBUTION
URINARY TRACT
VERTEBRATES