Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Induction of dihydrolipoamide dehydrogenase in differentiating 3T3-L1 cells

Thesis/Dissertation ·
OSTI ID:6905475
The activity and turnover of dihydrolipoamide dehydrogenase (E/sub 3/), the common component of the 3 ..cap alpha..-ketoacid dehydrogenase complexes, were measured during differentiation of 3T3-L1 preadipocytes into 3T3-L1 adipocytes. 3T3-L1 cells differentiate spontaneously or under the influence of hormones and chemicals into cells with many of the biological and biochemical features of adipocytes. The specific activity of E/sub 3/ increased approximately 3- to 4-fold following treatment with insulin, dexamethasone, and 1-methyl-3-isobutyl xanthine for 48 h, and insulin alone thereafter. Antibody to E/sub 3/ quantitatively precipitated the enzyme from 3T3-L1 adipocytes. Immunoprecipitation and gel electrophoresis showed an approximate 3-fold increase in E/sub 3/ protein from the adipocytes as compared to the same number of preadipocytes. Pulse labelling with L-(/sup 35/S)methionine showed a 3.5-fold increase in the relative rate of synthesis of E/sub 3/ in the 3T3-L1 adipocytes compared to the preadipocytes. In contrast, the apparent half-life of E/sub 3/ in preadipocytes was greater than or equal to that in adipocytes. Therefore, the increase in specific activity of E/sub 3/ in 3T3-L1 adipocytes results from an increased rate of enzyme synthesis.
Research Organization:
Case Western Reserve Univ., Cleveland, OH (USA)
OSTI ID:
6905475
Country of Publication:
United States
Language:
English

Similar Records

Induction of lipoamide dehydrogenase (E/sub 3/) in 3T3-L1 cells during differentiation
Conference · Fri May 01 00:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:6029237
Insulin regulation of protein biosynthesis in differentiated 3T3 adipocytes. Regulation of glyceraldehyde-3-phosphate dehydrogenase
Journal Article · Sat Oct 05 00:00:00 EDT 1985 · J. Biol. Chem.; (United States) · OSTI ID:6235749
Aculeatin, a coumarin derived from Toddalia asiatica (L.) Lam., enhances differentiation and lipolysis of 3T3-L1 adipocytes
Journal Article · Fri Oct 31 00:00:00 EDT 2014 · Biochemical and Biophysical Research Communications · OSTI ID:22416815

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AMINO ACIDS
ANIMAL CELLS
AROMATICS
AZAARENES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL HALF-LIFE
CARBOXYLIC ACIDS
CELL DIFFERENTIATION
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
DEXAMETHASONE
DRUGS
ENZYME ACTIVITY
ENZYME IMMUNOASSAY
ENZYME INDUCTION
ENZYMES
EVEN-ODD NUCLEI
FAT CELLS
FIBROBLASTS
GENE REGULATION
GLUCOCORTICOIDS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
IMMUNOASSAY
INSULIN
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
LIGHT NUCLEI
LIPOTROPIC FACTORS
METHIONINE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDOREDUCTASES
PEPTIDE HORMONES
PREGNANES
PURINES
RADIOISOTOPES
SOMATIC CELLS
STEROIDS
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
XANTHINES