Induction of lipoamide dehydrogenase (E/sub 3/) in 3T3-L1 cells during differentiation

Conference · Fri May 01 04:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6029237

Carothers, D J; Pons, G; Patel, M S

The authors have shown previously that the activity of the pyruvate dehydrogenase complex (PDC) increased several fold during differentiation of 3T3-L1 preadipocytes into adipocytes. The increase in total activity correlated with coordinated increases in the relative rates of synthesis (RRS) of ..cap alpha.. and ..beta.. subunits of the pyruvate dehydrogenase component. They now report investigations of the changes in the activity of E/sub 3/, another component of PDC, in 3T3-L1 cells during differentiation initiated by exposing cells to insulin, dexamethasone and 1-methyl-3-isobutylxanthine for 48 h followed by continued exposure to insulin alone. The specific activity (SA) of E/sub 3/ increased 3 to 4-fold during the differentiation. The amount of immunoprecipitable E/sub 3/ protein similarly increased in the differentiated cells. Immunotitrations of E/sub 3/ indicated that the SA of E/sub 3/ remained the same in both cell types. Pulse labeling of cells with /sup 35/S-methionine revealed a 3.5-fold increase in the RRS of E/sub 3/ in adipocytes compared to preadipocytes. The apparent degradation rates of E/sub 3/ were not significantly different in the two cells types. E/sub 3/ mRNA content, measured using an E/sub 3/ cDNA clone, was also increased during the differentiation. The increase in the RRS of E/sub 3/ is the primary determinant for increased enzyme protein in adipocytes.

• Research Organization: Case Western Reserve Univ. School of Medicine, Cleveland, OH
• OSTI ID: 6029237
• Report Number(s): CONF-870644-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AMINO ACIDS
ANIMAL CELLS
AROMATICS
AZAARENES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
CARBOXYLIC ACIDS
CELL DIFFERENTIATION
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
DEXAMETHASONE
DRUGS
ENZYME ACTIVITY
ENZYME INDUCTION
ENZYMES
EVEN-ODD NUCLEI
FAT CELLS
FIBROBLASTS
FUNCTIONS
GENE REGULATION
GLUCOCORTICOIDS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
INSULIN
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
LIGHT NUCLEI
LIPOTROPIC FACTORS
METHIONINE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDOREDUCTASES
PEPTIDE HORMONES
PREGNANES
PURINES
RADIOISOTOPES
SOMATIC CELLS
STEROIDS
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
XANTHINES