Studies on the asparagine-linked oligosaccharides from cartilage-specific proteoglycan

Thesis/Dissertation · Thu Jan 01 04:00:00 EST 1987

OSTI ID:6905471

Cioffi, L C

Chondrocytes synthesize and secrete a cartilage-specific proteoglycan (PG-H) as one of their major products. This proteoglycan has attached to it several types of carbohydrate chains, including chondroitin sulfate, keratan sulfate, O-linked oligosaccharides, and asparagine-linked oligosaccharides. The asparagine-linked oligosaccharides found on PG-H were investigated in these studies. Methodology was developed for the isolation and separation of standard of standard complex and high mannose type oligosaccharides. This included digesting glycoproteins with N-glycanase and separation of the oligosaccharides according to type by concanavalin-A lectin chromatography. The different oligosaccharide types were then analyzed by high pressure liquid chromatography. This methodology was used in the subsequent studies on the PG-H asparagine-linked oligosaccharides. Initially, the asparagine-linked oligosaccharides recovered from the culture medium (CM) and cell-associated (Ma) fractions of PG-H from of tibial chondrocytes were labeled with (/sup 3/H)-mannose and the oligosaccharides were isolated and analyzed.

• Research Organization: Illinois Univ., Urbana (USA)
• OSTI ID: 6905471
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
CARBOHYDRATES
CHEMICAL COMPOSITION
CHROMATOGRAPHY
GLUCOPROTEINS
GLYCOPROTEINS
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MANNOSE
MOLECULAR STRUCTURE
MONOSACCHARIDES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PROTEINS
SACCHARIDES
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS