Transforming growth factor-beta 1 stimulates synthesis of proteoglycan aggregates in calf articular cartilage organ cultures

Journal Article · Sun Mar 31 23:00:00 EST 1991 · Archives of Biochemistry and Biophysics; (United States)

DOI:https://doi.org/10.1016/0003-9861(91)90013-9· OSTI ID:5080104

Morales, T I ^[1]

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1. Bone Research Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, MD (United States)

Previous work showed that transforming growth factor-beta 1 (TGF-beta 1), added alone to bovine cartilage organ cultures, stimulated (35S)sulfate incorporation into macromolecular material but did not investigate the fidelity of the stimulated system to maintain synthesis of cartilage-type proteoglycans. This paper provides evidence that chondrocytes synthesize the appropriate proteoglycan matrix under TGF-beta 1 stimulation: (1) there is a coordinated increase in hyaluronic acid and proteoglycan monomer synthesis, (2) link-stable proteoglycan aggregates are assembled, (3) the hybrid chondroitin sulfate/keratan sulfate monomeric species is synthesized, and (4) there is an increase in protein core synthesis. Some variation in glycosylation patterns was observed when proteoglycans synthesized under TGF-beta 1 stimulation were compared to those synthesized under basal conditions. Thus comparing TGF-beta 1 to basal samples respectively, the monomers were larger (Kav on Sepharose CL-2B = 0.29 vs 0.41), the chondroitin sulfate chains were longer by approximately 3.5 kDa, the percentage of total glycosaminoglycan in keratan sulfate increased slightly from approximately 4% (basal) to approximately 6%, and the unsulfated disaccharide decreased from 28% (basal) to 12%. All of these variations are in the direction of a more anionic proteoglycan. Since the ability of proteoglycans to confer resiliency to the cartilage matrix is directly related to their anionic nature, these changes would presumably have a beneficial effect on tissue function.

• OSTI ID: 5080104
• Journal Information: Archives of Biochemistry and Biophysics; (United States), Journal Name: Archives of Biochemistry and Biophysics; (United States) Vol. 286:1; ISSN ABBIA; ISSN 0003-9861
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BODY
CALVES
CARBOHYDRATES
CARTILAGE
CATTLE
CONNECTIVE TISSUE
DAYS LIVING RADIOISOTOPES
DISACCHARIDES
DOMESTIC ANIMALS
EVEN-ODD NUCLEI
GLYCOPROTEINS
GROWTH FACTORS
HYALURONIC ACID
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAMMALS
MITOGENS
MUCOPOLYSACCHARIDES
NUCLEI
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RUMINANTS
SACCHARIDES
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
TISSUE CULTURES
TISSUES
TRACER TECHNIQUES
VERTEBRATES