Characterization of the phase dependent pulmonary response following irritant inhalation exposure to nitrogen dioxide gas
The present study utilized NO{sub 2} to fingerprint the biochemical reaction of the pulmonary compartment to oxidative damage and to correlate this with histopathology following acute and subacute exposures to NO{sub 2}. Acute exposure to NO{sub 2} produced dose-dependent immediate increases in the nonenzymatic parameters of pulmonary protein content, protease inhibitor activity and lung weight. The enzymatic activities of lactate dehydrogenase (LDH), choline kinase and beta-glucuronidase were elevated by two days following acute exposure. All of the above parameters were elevated following subacute exposure, however, nonenzymatic manifestations were attenuated with respect to enzymatic alterations. Hydroxyurea-induced granulocytopenia attenuated the increases in activities of LDH and beta-glucuronidase following acute, but not subacute exposures. Cycloheximide-induced protein synthesis inhibition decrease the LDH and beta-glucuronidase response to NO{sub 2} without altering the increases in protein content or protease inhibitor activity.
- Research Organization:
- Tulane Univ., New Orleans, LA (USA)
- OSTI ID:
- 6905199
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GLUCURONIDASE
ENZYME ACTIVITY
LACTATE DEHYDROGENASE
LUNGS
SENSITIVITY
NITROGEN DIOXIDE
TOXICITY
PHOSPHOTRANSFERASES
ACUTE EXPOSURE
DOSE-RESPONSE RELATIONSHIPS
ENZYME INHIBITORS
INHALATION
BODY
CHALCOGENIDES
ENZYMES
GLYCOSYL HYDROLASES
HEMIACETAL DEHYDROGENASES
HYDROLASES
INTAKE
NITROGEN COMPOUNDS
NITROGEN OXIDES
O-GLYCOSYL HYDROLASES
ORGANS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
RESPIRATORY SYSTEM
TRANSFERASES
560300* - Chemicals Metabolism & Toxicology