Characterization of the phase dependent pulmonary response following irritant inhalation exposure to nitrogen dioxide gas

Thesis/Dissertation · Fri Jan 01 04:00:00 EST 1988

OSTI ID:6905199

Siegel, P D

The present study utilized NO{sub 2} to fingerprint the biochemical reaction of the pulmonary compartment to oxidative damage and to correlate this with histopathology following acute and subacute exposures to NO{sub 2}. Acute exposure to NO{sub 2} produced dose-dependent immediate increases in the nonenzymatic parameters of pulmonary protein content, protease inhibitor activity and lung weight. The enzymatic activities of lactate dehydrogenase (LDH), choline kinase and beta-glucuronidase were elevated by two days following acute exposure. All of the above parameters were elevated following subacute exposure, however, nonenzymatic manifestations were attenuated with respect to enzymatic alterations. Hydroxyurea-induced granulocytopenia attenuated the increases in activities of LDH and beta-glucuronidase following acute, but not subacute exposures. Cycloheximide-induced protein synthesis inhibition decrease the LDH and beta-glucuronidase response to NO{sub 2} without altering the increases in protein content or protease inhibitor activity.

• Research Organization: Tulane Univ., New Orleans, LA (USA)
• OSTI ID: 6905199
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACUTE EXPOSURE
BODY
CHALCOGENIDES
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
GLUCURONIDASE
GLYCOSYL HYDROLASES
HEMIACETAL DEHYDROGENASES
HYDROLASES
INHALATION
INTAKE
LACTATE DEHYDROGENASE
LUNGS
NITROGEN COMPOUNDS
NITROGEN DIOXIDE
NITROGEN OXIDES
O-GLYCOSYL HYDROLASES
ORGANS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
RESPIRATORY SYSTEM
SENSITIVITY
TOXICITY
TRANSFERASES