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The megakaryocyte DNA content and platelet formation after the sublethal whole body irradiation of rats

Journal Article · · Blood; (United States)
OSTI ID:6904592

The DNA content of rat bone marrow megakaryocytes (MK) was studied by Feulgen photometry, following whole body irradiation with 2 Gy. The DNA measurements were preceded by acetylcholinesterase staining to avoid missing the smaller 2N-8N MK. The number of 2N-8N MK declined immediately following irradiation, whereas the number of 16N-64N MK remained normal for 4 days before decreasing. The number of 2N-8N and 16N-64N MK reached minimum around days 7 and 10, respectively, and thereafter increased to supranormal values at days 14 and 20, respectively. Platelet production, measured by /sup 35/S incorporation into platelets, increased during the first 4 days, then decreased to minimum about day 10. A rise to supranormal values was present at day 20. All values were about normal 30 days after exposure. The observed pattern may be explained as follows: Most of the 16N-64N MK survive the applied dose and maintain their ability to produce platelets. Some of the 2N-4N and 8N MK survive irradiation and transform into platelet-producing MK. No influx of cells from the MK stem cell compartment into the MK compartment can be observed before day 7 after irradiation. One explanation for this time lag may be that thrombocytopenia, which does not occur before then, is an essential stimulus for MK stem cell activation.

Research Organization:
Department of Haematology, Norwegian Radium Hospital, Oslo, Norway
OSTI ID:
6904592
Journal Information:
Blood; (United States), Journal Name: Blood; (United States) Vol. 63:4; ISSN BLOOA
Country of Publication:
United States
Language:
English

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Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BLOOD COUNT
BLOOD FORMATION
BLOOD PLATELETS
BODY FLUIDS
BONE MARROW CELLS
CONNECTIVE TISSUE CELLS
DNA
EXTERNAL IRRADIATION
IRRADIATION
MAMMALS
MATERIALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RATS
RODENTS
SOMATIC CELLS
VERTEBRATES
WHOLE-BODY IRRADIATION