High-resolution genomic mapping of the three human replication protein A genes (RPA1, RPA2, and RPA3)

Journal Article · Tue Mar 15 04:00:00 EST 1994 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1994.1161· OSTI ID:6903901

Umbricht, C B; Kelly, T J; Griffin, C A; Hawkins, A L ^[1]; Grzeschik, K H ^[2]; O'Connell, P; Leach, R ^[3]; Green, E D ^[4]

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1. Johns Hopkins Univ. School of Medicine, Baltimore, MD (United States)
2. Klinikum der Philipps-Universitaet Marburg (Germany)
3. Univ. of Texas Health Science Center, San Antonio, TX (United States)
4. Washington Univ. of School, St. Louis, MO (United States)

Human replication protein A (RPA) is a three-subunit protein that plays a central role in eukaryotic DNA replication, homologous recombination, and excision repair. The authors have previously reported the cloning and bacterial overexpression of the three RPA genes and have mapped them to chromosome 1 (RPA2), chromosome 7 (RPA3), and chromosome 17 (RP1). They have now obtained yeast strains with artificial chromosomes carrying the three human RPA genes and report the more detailed genomic mapping of RPA. RPA1 was mapped to chromosome 17p13.3 using a combination of PCR amplification of somatic cell hybrids and radiation hybrids containing chromosome 17 fragments. RPA2 was mapped to chromosome 1p35 by PCR amplification of somatic cell hybrids of chromosome 1 and by fluorescence in situ hybridization. RPA3 was mapped to chromosome 7p22 by Southern analysis and PCR amplification of somatic cell hybrids of chromosome 7 as well as fluorescence in situ hybridization. Since RPA is an essential component of major metabolic events affecting DNA, the physical mapping of the genes for it may help elucidate the biochemical basis of genetic disorders involving DNA metabolism. 73 refs., 8 figs., 1 tab.

• OSTI ID: 6903901
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 20:2; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHROMOSOMES
DNA HYBRIDIZATION
DNA REPAIR
DNA REPLICATION
EXCISION REPAIR
GENE AMPLIFICATION
GENE RECOMBINATION PROTEINS
GENE REGULATION
GENETIC MAPPING
HUMAN CHROMOSOME 1
HUMAN CHROMOSOME 17
HUMAN CHROMOSOME 7
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING
NUCLEIC ACID REPLICATION
POLYMERASE CHAIN REACTION
REPAIR