Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Anticoagulantly active heparan sulfate and proteoheparan sulfate from cloned bovine aortic endothelial cells

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6899011
; ; ;
Heparan sulfate chains and intact proteoheparans were isolated from cloned endothelial cells of bovine aortic intima. The cells were grown in vitro and metabolically labeled using Na/sub 2/(/sup 35/S)SO/sub 4/ and/or tritiated amino acids. The radiolabeled heparan chains (M/sub r/ approx. = 30,000) and the proteoglycans were affinity fractionated on immobilized antithrombin and assayed for anticoagulant activity by radioimmunoassay for thrombin-antithrombin complex. About 1% of the heparan chains and proteoheparan bound antithrombin and accounted for >99% and >85% of the anticoagulant activity, respectively. Comparison of the bound and unbound fractions demonstrated a 6000-fold increase in specific activity for the heparan chains and a >1000-fold increase in specific activity for the proteoglycan. The disaccharide compositions of nitrous acid-cleaved heparan sulfate chains were analyzed by ion-exchange HPLC and found to have about a 4-fold enhancement in the bound fraction of GlcA ..-->.. GlcN-3-0-SO/sub 3/. This disaccharide has been shown previously to be a marker for the antithrombin binding domain in commercial heparin. These results demonstrate that bovine aortic endothelial cells synthesize heparan sulfate and proteoheparan which exhibit anticoagulant activity indistinguishable from heparin, and that the active species contains disaccharides which constitute the unique antithrombin binding domain of heparin.
Research Organization:
MIT, Cambridge
OSTI ID:
6899011
Report Number(s):
CONF-8606151-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
Country of Publication:
United States
Language:
English

Similar Records

Heparin sequences in the heparan sulfate chains of an endothelial cell proteoglycan
Journal Article · Mon Jun 01 00:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States) · OSTI ID:5303064
Heparin releases heparan sulfate from the cell surface
Journal Article · Mon Oct 24 00:00:00 EDT 1977 · Biochem. Biophys. Res. Commun.; (United States) · OSTI ID:5039568
Human recombinant interleukin-1 beta- and tumor necrosis factor alpha-mediated suppression of heparin-like compounds on cultured porcine aortic endothelial cells
Journal Article · Sat Sep 01 00:00:00 EDT 1990 · Journal of Cellular Physiology; (USA) · OSTI ID:6511681

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
AMINES
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
ANTICOAGULANTS
AORTA
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BLOOD COAGULATION
BLOOD VESSELS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CATTLE
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
DRUGS
ENDOTHELIUM
EVEN-ODD NUCLEI
FRACTIONATION
HEMATOLOGIC AGENTS
HEPARIN
IMMUNOASSAY
IMMUNOLOGY
ION EXCHANGE CHROMATOGRAPHY
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAMMALS
MUCOPOLYSACCHARIDES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
POLYSACCHARIDES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
REACTION KINETICS
RUMINANTS
SACCHARIDES
SEPARATION PROCESSES
STRUCTURE-ACTIVITY RELATIONSHIPS
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
TISSUES
TRACER TECHNIQUES
VERTEBRATES