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Ethanol production by Zymomonas mobilis in fed-batch fermentations

Journal Article · · Biotechnol. Bioeng.; (United States)
OSTI ID:6897956
The production of ethanol by Zymomonas mobilis NRRL B-4286 was studied in fed-batch cultures. Initial percent (w/v) glucose, rate of feed, and quantity of 50% (w/v) glucose feed were varied. Glucose inhibition of growth rate occurred at concentrations greater than 8% (w/v). Feed was begun after 4 hours incubation. Feed volume was ca. 36% of starting batch volume to get ca. 10% (w/v) ethanol at harvest. The range of feed rates studied varied from 16% batch volume/h (glucose concentration increased to an inhibitory level) to 4% batch volume/h (glucose concentration dropped rapidly to zero and was limiting). Increasing feed volume to 46% of starting volume at the best feed rate (ca. 10% feed volume/h) increased final ethanol concentration to 11.3% (w/v). However, the resultant increase in fermentation time from ca. 21 to 29 h decreased ethanol volumetric productivity from 5.2-4.6 g/L h. (Refs. 9).
Research Organization:
Northern Regional Research Center, Agricultural Research Service, US Dept of Agriculture, Peoria, IL 61604
OSTI ID:
6897956
Journal Information:
Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 26:3; ISSN BIBIA
Country of Publication:
United States
Language:
English