Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Ethanol production by Zymomonas mobilis in fed-batch fermentations

Journal Article · · Biotechnol. Bioeng.; (United States)
The production of ethanol by Zymomonas mobilis NRRLB-4286 was studied in fed-batch cultures. Initial percent (w/v) glucose, rate of feed, and quantity of 50% (w.v) glucoses feed were varied. Glucose inhibition of growth rate occurred at concentrations greater than 85 (w.v). Feed was begun after 4 h incubation. Feed volume was ca. 36% of starting batch volume to get ca. 10% (w/v) ethanol at harvest. The range of feed rates studied varied from 16% batch volume/h (glucose concentration increased to an inhibitory level) to 4% batch volume/h (glucose concentration dropped rapidly to zero and was limiting). Increasing feed volume to 46% of starting volume at the best feed rate (ca. 10% feed volume/h) increased final ethanol concentration to 11.3% (w/v). However, the resultant increase in fermentation time from ca. 21 to 29 h decreased ethanol volumetric productivity from 5.2 to 4.6 g/L h.
Research Organization:
Dept. of Agriculture, Peoria, IL
OSTI ID:
6764403
Journal Information:
Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 26:3; ISSN BIBIA
Country of Publication:
United States
Language:
English