Identification of a novel inositol phosphate recognition site: Specific (3H)inositol hexakisphosphate binding to brain regions and cerebellar membranes
Journal Article
·
· Biochemical and Biophysical Research Communications; (USA)
- MRC Molecular Neurobiology Unit, Cambridge (England)
(3H)Inositol hexakisphosphate (InsP6) binds with a heterogeneous distribution to frozen sections of unfixed rat brain and is displaced by unlabelled InsP6. The pattern of binding correlates with binding to neuronal cell bodies. (3H)InsP6 binding to cerebellar membranes has been further characterized, is reversible, and saturable, and exhibits high specificity for inositol polyphosphates. The IC50 for competition by unlabelled InsP6 is approximately 100nM, whereas inositol 1,3,4,5,6 pentakisphosphate (Ins(13456)P5), inositol 1,3,4,5 tetrakisphosphate (Ins(1345)P4), and inositol 1,4,5 trisphosphate (Ins(145)P3) bind with an affinity at least one order of magnitude lower. (3H)InsP6 binding is clearly distinct from previously characterized Ins(145)P3 (ref. 1, 2) and Ins(1345)P4 (ref. 3) binding, both in terms of pharmacology and brain distribution.
- OSTI ID:
- 6897128
- Journal Information:
- Biochemical and Biophysical Research Communications; (USA), Journal Name: Biochemical and Biophysical Research Communications; (USA) Vol. 167:2; ISSN BBRCA; ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CATTLE
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
CEREBELLUM
DOMESTIC ANIMALS
ESTERS
HYDROGEN COMPOUNDS
KINETICS
LIPIDS
MAMMALS
MEMBRANES
NERVE CELLS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
RATS
REACTION KINETICS
RODENTS
RUMINANTS
SOMATIC CELLS
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CATTLE
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
CEREBELLUM
DOMESTIC ANIMALS
ESTERS
HYDROGEN COMPOUNDS
KINETICS
LIPIDS
MAMMALS
MEMBRANES
NERVE CELLS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
RATS
REACTION KINETICS
RODENTS
RUMINANTS
SOMATIC CELLS
TRITIUM COMPOUNDS
VERTEBRATES