Use of positive ion fast atom bombardment mass spectrometry for rapid identification of a bile alcohol glucuronide isolated from cerebrotendinous xanthomatosis patients

Journal Article · Thu Feb 01 04:00:00 EST 1990 · Steroids; (USA)

DOI:https://doi.org/10.1016/0039-128X(90)90028-A· OSTI ID:6896155

Dayal, B; Salen, G; Tint, G S; Shefer, S; Benz, S W ^[1]

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1. UMDNJ-New Jersey Medical School, Newark (USA)

The identification of a major biliary and plasma bile alcohol glucuronide, 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha, 25-tetrol-3-0-beta-D-glucuronide, present in cerebrotendinous xanthomatosis (CTX) patients, was investigated by positive ion fast atom bombardment mass spectrometry (FAB-MS). The spectrum was characterized by abundant ions formed by attachment of a proton, (M + H)+, or of alkali ions, (M + Na)+ and (M + 39K)+, to the glucuronide salt. These ions allowed an unambiguous deduction of the molecular weight of the sample. It is suggested that FAB-MS could be used in the rapid diagnosis of CTX.

• OSTI ID: 6896155
• Journal Information: Steroids; (USA), Journal Name: Steroids; (USA) Vol. 55:2; ISSN 0039-128X; ISSN STEDA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALDEHYDES
ALKALI METAL ISOTOPES
ANIMALS
BARYONS
BILIARY TRACT
CARBOXYLIC ACIDS
CHOLESTEROL
DIAGNOSIS
DIGESTIVE SYSTEM
DIGESTIVE SYSTEM DISEASES
DISEASES
ELECTRONS
ELEMENTARY PARTICLES
FERMIONS
GLUCURONIC ACID
HADRONS
HYDROXY ACIDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LEPTONS
LIGHT NUCLEI
MAMMALS
MAN
MASS SPECTRA
NUCLEI
NUCLEONS
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
POTASSIUM 39
POTASSIUM ISOTOPES
PRIMATES
PROTONS
SPECTRA
STABLE ISOTOPES
STEROIDS
STEROLS
TRACER TECHNIQUES
VERTEBRATES