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Metabolism of C26 bile alcohols in the bullfrog, Rana catesbeiana

Journal Article · · Steroids; (United States)
; ; ;

Metabolism of C26 bile alcohols in the bullfrog, Rana catesbeiana, was studied. (24-14C)-24-Dehydro-26-deoxy-5 beta-ranol (3 alpha,7 alpha,12 alpha-trihydroxy-27-nor-5 beta-cholestan-24-one) was chemically synthesized from (24-14C)cholic acid and incubated with bullfrog liver homogenate fortified with NADPH. 24-Dehydro-26-deoxy-5 beta-ranol was shown to be converted into both 26-deoxy-5 beta-ranol and 24-epi-26-deoxy-5 beta-ranol ((24S)- and (24R)-27-nor-5 beta-cholestane-3 alpha,7 alpha,12 alpha,24-tetrols) in addition to 5 beta-ranol ((24R)-27-nor-5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,26-pentol), which is the major bile alcohol of the bullfrog. (24-3H)-26-Deoxy-5 beta-ranol and (24-3H)-24-epi-26-deoxy-5 beta-ranol were prepared from 24-dehydro-26-deoxy-5 beta-ranol by reduction with sodium (3H) borohydride and administered respectively to two each of four bullfrogs by intraperitoneal injection. After 24 h, labeled 5 beta-ranol was isolated from the bile of the bullfrogs that received (24-3H)-26-deoxy-5 beta-ranol. In contrast little if any radioactivity could be detected in 5 beta-ranol or its 24-epimer after administration of (24-3H)-24-epi-26-deoxy-5 beta-ranol.

Research Organization:
Hiroshima Prefectural Research Center for Environmental Science (Japan)
OSTI ID:
6463815
Journal Information:
Steroids; (United States), Journal Name: Steroids; (United States) Vol. 51:3-4; ISSN STEDA
Country of Publication:
United States
Language:
English

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Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
ASCORBIC ACID
BILE ACIDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
DRUGS
ENZYMES
ESTRADIOL
ESTRANES
ESTROGENS
FROGS
GLUTATHIONE
HORMONES
HYDROXY COMPOUNDS
HYDROXYLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RATS
RODENTS
STEROID HORMONES
STEROIDS
STEROLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TYROSINASE
VERTEBRATES
VITAMINS