Chronic ethanol consumption alters the selective usage of phosphatidylethanolamine molecular species by methyltransferases

Conference · Wed Jan 01 00:00:00 EST 1992 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:6894093

Ellingson, J S; Pukys, T; Rubin, E ^[1]

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1. Thomas Jefferson Univ., Philadelphia, PA (United States)

The authors have examined the effects of chronic ethanol consumption on phospholipid methyltransferases, which may play a special role by synthesizing phosphatidylcholine (PC) molecules containing predominantly polyunsaturated fatty acids. Rat liver microsomes from adenosylmethionine to convert endogenous phoshatidylethanolamine (PE) to radiolabeled PC, which was separated into its individual molecular species by reversed-phase HPLC. To assess the selective usage of PE molecular species for methylation, the authors determined the mole % of the PE molecular species in microsomes from control and ethanol-fed rats. Chronic ethanol consumption increased the selective usage of phospholipid molecular species containing palmitic acid combined with arachidonic acid or docosahexaenoic acid, whereas it did not affect the use of the corresponding stearic acid species. These results suggest that the long term interference with cellular physiology by altering the metabolism of a specific metabolic pool of molecular species is a mechanism by which chronic ethanol consumption could exert adverse effects of the liver.

• OSTI ID: 6894093
• Report Number(s): CONF-9202109-; CODEN: FAJOEC
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 6:1; Conference: American Society for Biochemistry and Molecular Biology and Biophysical Society joint meeting, Houston, TX (United States), 9-13 Feb 1992; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ETHANOL
BIOLOGICAL EFFECTS
METHYL TRANSFERASES
ENZYME ACTIVITY
PHOSPHOLIPIDS
BIOSYNTHESIS
BIOLOGICAL FUNCTIONS
CARBOXYLIC ACIDS
CHRONIC EXPOSURE
LIQUID COLUMN CHROMATOGRAPHY
LIVER
METABOLISM
METHYLATION
MICROSOMES
RADIOISOTOPES
RATS
TRACER TECHNIQUES
ALCOHOLS
ANIMALS
BODY
CARBON-GROUP TRANSFERASES
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DIGESTIVE SYSTEM
ENZYMES
ESTERS
GLANDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIPIDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PROTEINS
RIBOSOMES
RODENTS
SEPARATION PROCESSES
SYNTHESIS
TRANSFERASES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology