Prostaglandin synthetase dependent activation of 7,8-dihydro-7,8-dihydroxy-benzo(a)pyrene to mutagenic derivatives

Journal Article · Mon May 15 04:00:00 EDT 1978 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(78)90597-1· OSTI ID:6893482

Marnett, L J; Reed, G A; Dennison, D J

The combination of arachidonic acid and a Tween 20 solubilized enzyme preparation from sheep seminal vesicles converts 7,8-dihydro-7,8-dihydroxy-benzo(a)pyrene to derivatives strongly mutagenic to Salmonella typhimurium tester strain TA 98. Under similar conditions no activation of benzo(a)pyrene, 4,5-dihydro-4,5-dihydroxy-benzo(a)pyrene, or 9,10-dihydro-9,10-dihydroxy-benzo(a)pyrene occurs. The activation of 7,8-dihydro-7,8-dihydroxy-benzo(a)pyrene is markedly reduced by the omission of arachidonic acid and is inhibited by the prostaglandin synthetase inhibitor indomethacin. 100 ..mu..M butylated hydroxyanisole is also an effective inhibitor. This is the first report of the metabolic activation of 7,8-dihydro-7,8-dihydroxy-benzo(a)pyrene by an enzyme system distinct from the mixed-function oxidases.

• Research Organization: Wayne State Univ., Detroit
• OSTI ID: 6893482
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 82:1; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

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550500* -- Metabolism
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANISOLE
ARACHIDONIC ACID
AROMATICS
BACTERIA
BENZOPYRENE
BODY
CARBOXYLIC ACIDS
CARCINOGENS
CHEMICAL ACTIVATION
CONDENSED AROMATICS
DOMESTIC ANIMALS
ENZYMES
ETHERS
HYDROCARBONS
HYDROXY COMPOUNDS
INHIBITION
LIGASES
MALE GENITALS
MAMMALS
METABOLISM
MICROORGANISMS
MONOCARBOXYLIC ACIDS
MUTAGENS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
OXIDASES
OXIDOREDUCTASES
PROSTAGLANDINS
RUMINANTS
SALMONELLA
SHEEP
VERTEBRATES