Loss of proliferative capacity in immunohemopoietic stem cells caused by serial transplantation rather than aging

Journal Article · Sun Jan 01 04:00:00 EST 1978 · J. Exp. Med.; (United States)

DOI:https://doi.org/10.1084/jem.147.5.1526· OSTI ID:6893345

Harrison, D E; Astle, C M; Delaittre, J A

Marrow stem cell lines from old donors and those from young controls gave equally rapid rates of colony growth on spleens of irradiated mice. Old and young stem cell lines competed equally well with chromosomally marked marrow stem cells from a young donor in producing cell types that are stimulated by bleeding; old cells competed 70 percent as well as young in producing cell types stimulated by phytohemagglutinin (PHA) in vitro. After a single serial transplantation, the rates of colony growth declined 1.5 to 2.5-fold, and the ability to compete declined 2- to 4-fold for bleeding-stimulated and 4- to 10-fold for PHA-stimulated cells. Thus, immediate stem cell proliferative capacities decline much more after one serial transplantation than after a lifetime of normal function.

• Research Organization: Jackson Lab., Bar Harbor, ME
• OSTI ID: 6893345
• Journal Information: J. Exp. Med.; (United States), Journal Name: J. Exp. Med.; (United States) Vol. 147:5; ISSN JEMEA
• Country of Publication: United States
• Language: English

Related Subjects

551000* -- Physiological Systems
560152 -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOCHEMISTRY
BODY
BONE MARROW
CELL DIVISION
CHEMISTRY
COMPARATIVE EVALUATIONS
HEMATOPOIETIC SYSTEM
MAMMALS
MICE
ORGANS
PHYSIOLOGY
RADIATION EFFECTS
RODENTS
SPLEEN
TISSUES
TRANSPLANTS
VERTEBRATES