DNA repair and cell repair: are they related

Conference · Sun Jul 01 04:00:00 EDT 1979 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

OSTI ID:6881615

Elkind, M M

Evidence of damage-repair processes is reviewed in connection with 3 types of cytotoxic cell treatments: x-radiation; far-ultraviolet (uv) (254 nm) light; and fluorescent light (FL) exposure of cells that had been grown in the presence of 5-bromodeoxyuridine (BUdR). In each case, the processes are characterized relative to cell survival and loss of DNA integrity, i.e. the presence of single-strand breaks evident under alkaline conditions and dimers containing thymine. From quantitative data of the induction of both types of end points, the number of DNA lesions are compared that are produced by a D/sub o/ dose; that is, a dose that reduces survival by the factor 1/e (approx. = 0.37). The number of single-strand breaks for the treatments BUdR/FL, x-rays, and far-uv are respectively approx. 50,000, approx. 1000, and approx. 100. From such data, and upper-limit estimates of the persistence of breaks in x-irradiated surviving cells, it is concluded that: (1) x-ray survivors repair a large number of DNA lesions; and (2) x-ray lethality results from damage registered in a small fraction of the genome and/or the misrepair of supernumerary DNA lesions.

• Research Organization: Argonne National Lab., IL
• OSTI ID: 6881615
• Conference Information: Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Journal Volume: 5:7
• Country of Publication: United States
• Language: English

Related Subjects

560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
560121 -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL MODELS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BROMOURACILS
BUDR
CELL KILLING
COMPARATIVE EVALUATIONS
DNA
DRUGS
ELECTROMAGNETIC RADIATION
FLUORESCENT LAMPS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IONIZING RADIATIONS
LIGHT BULBS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
RIBOSIDES
STRAND BREAKS
ULTRAVIOLET RADIATION
URACILS
VISIBLE RADIATION
X RADIATION