Detection of genetic variation with radioactive ligands. I. Electrophoretic screening of plasma proteins with a selected panel of compounds

Journal Article · Tue Nov 01 04:00:00 EST 1977 · Am. J. Hum. Genet.; (United States)

OSTI ID:6880919

Cavalli-Sforza, L L; Daiger, S P; Rummel, D P

To detect new genetic variation in human plasma proteins, a panel of 63 radioactive substances were screened as potential radioligands using polyacrylamide gel electrophoresis (PAGE) and autoradiography. Vitamins, hormones, drugs, amino acids, purines, pyrimidines, sugars and lipids labeled with /sup 14/C or other radionuclides were among those substances tested. A majority bound to albumin and a smaller fraction to prealbumins and lipoproteins. Several vitamins and hormones bound to specific alpha and beta globulins. (1) Electrophoretic polymorphisms of vitamin D-binding protein (group-specific component), a vitamin B12-binding protein (transcobalamin II), and thyroxine-binding alpha-globulin are described elsewhere; (2) testosterone-binding beta-globulin (TeBG) showed an electrophoretic polymorphism in Caucasians and a possible deficiency allele; (3) transcortin showed an electrophoretic doublet in all persons tested but no electrophoretic variation; (4) a protein binding derivative of norepinephrine or epinephrine was identified as transferrin; (5) a nonpolymorphic protein running cathodal to albumin and binding a derivative of riboflavin was tentatively identified as a fraction of albumin with mobility altered as a result of interaction with the ligand.

• Research Organization: Stanford Univ., CA
• OSTI ID: 6880919
• Journal Information: Am. J. Hum. Genet.; (United States), Journal Name: Am. J. Hum. Genet.; (United States) Vol. 29:6; ISSN AJHGA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL VARIABILITY
BIOLOGY
BLOOD
BODY FLUIDS
CARBON 14
CARBON ISOTOPES
DIAGNOSTIC USES
ELECTROPHORESIS
EVEN-EVEN NUCLEI
GENETIC VARIABILITY
GENETICS
ISOTOPES
LABELLED COMPOUNDS
LIGANDS
LIGHT NUCLEI
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
USES
YEARS LIVING RADIOISOTOPES