Looping out and deletion mechanism for the immunoglobulin heavy-chain class switch
In the mouse pre-B-cell line 18-81, cells can switch production in vitro from immunoglobulin ..mu.. chain to ..gamma..2b chain. The gene encoding the ..gamma..2b chain is created by a rearrangement of the ..mu.. gene. This rearrangement always takes place within a homolog. In cells with a ..gamma..2b gene, most of the time the gene segment encoding the constant region of the ..mu.. chain is deleted, but often the rearrangement leads to cells that produce no immunoglobulin, and all DNA sequences are retained. The latter result is due to an inversion. Inversions exclude the unequal sister chromatid exchange model of the heavy-chain class switch. Looping out is an intermediate step in the process of generating an inversion. These finding demonstrate that the switch rearrangement occurs by looping out and deletion.
- Research Organization:
- Univ. of California, San Francisco (USA)
- DOE Contract Number:
- AC03-76SF01012
- OSTI ID:
- 6879720
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 85:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHROMOSOMAL ABERRATIONS
MOLECULAR BIOLOGY
IMMUNOGLOBULINS
BIOSYNTHESIS
DNA SEQUENCING
ELECTROPHORESIS
IN VITRO
LYMPHOCYTES
METHIONINE
MICE
SISTER CHROMATID EXCHANGES
SULFUR 35
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
GLOBULINS
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
MATERIALS
MUTATIONS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
SULFUR ISOTOPES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques