High rates of deletions in the constant region segment of the immunoglobulin. mu. gene
Spontaneous deletions at the immunoglobulin heavy-chain locus are frequently found in myelomas, hybridomas, and pre-B-cell lines. The authors have measured the rates for large and small deletions within the constant-region gene segment for ..mu.. chain in a pre-B-cell line. The large deletions, which include the entire first and second exons, occurred at a rate of 1.7 x 10/sup -5/ per cell generation. The small deletions, which span a few base pairs, occurred at a rate of 1.4 x 10/sup -7/ per cell generation. The rate for the reversion of a termination codon in the second exon is even less than that for the small deletions and is 1000 times lower than the reversion rate that had been determined for the variable-region gene segment. Therefore, the variable-region gene segment is likely to be the preferred target for hypermutation.
- Research Organization:
- Univ. of California, San Francisco (USA)
- DOE Contract Number:
- AC03-76SF01012
- OSTI ID:
- 5209364
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:14
- Country of Publication:
- United States
- Language:
- English
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GENOME MUTATIONS
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DNA SEQUENCING
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DNA-CLONING
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MAN
METHIONINE
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TUMOR CELLS
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
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BODY FLUIDS
CARBOXYLIC ACIDS
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CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
GLOBULINS
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
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NUCLEI
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ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PRIMATES
PROTEINS
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SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
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550201* - Biochemistry- Tracer Techniques