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Human placental Na/sup +/, K/sup +/-ATPase. cap alpha. subunit: cDNA cloning, tissue expression, DNA polymorphism, and chromosomal localization

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
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A 2.2-kilobase clone comprising a major portion of the coding sequence of the Na/sup +/, K/sup +/-ATPase ..cap alpha.. subunit was cloned from human placenta and its sequence was identical to that encoding the ..cap alpha.. subunit of human kidney and HeLa cells. Transfer blot analysis of the mRNA products of the Na/sup +/, K/sup +/-ATPase gene from various human tissues and cell lines revealed only one band (approx. = 4.7 kilobases) under low and high stringency washing conditions. The levels of expression in the tissues were intestine > placenta > liver > pancreas, and in the cell lines the levels were human erythroleukemia > butyrate-induced colon > colon > brain > HeLa cells. mRNA was undetectable in reticulocytes, consistent with the authors failure to detect positive clones in a size-selected ( > 2 kilobases) lambdagt11 reticulocyte cDNA library. DNA analysis revealed by a polymorphic EcoRI band and chromosome localization by flow sorting and in situ hybridization showed that the ..cap alpha.. subunit is on the short is on the short arm (band p11-p13) of chromosome 1.

Research Organization:
Univ. of California, San Francisco (USA)
OSTI ID:
6879523
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:22; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
AMINO ACID SEQUENCE
ANIMAL CELLS
ANIMALS
ATP-ASE
AUTORADIOGRAPHY
BODY
CHROMOSOMES
CLONING
DIGESTIVE SYSTEM
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
ENDOCRINE GLANDS
ENZYMES
FETAL MEMBRANES
GASTROINTESTINAL TRACT
GENETIC MAPPING
GLANDS
HELA CELLS
HYBRIDIZATION
HYDROLASES
INTESTINES
KIDNEYS
LIVER
MAMMALS
MAN
MAPPING
MEMBRANES
MESSENGER-RNA
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PHOSPHOHYDROLASES
PLACENTA
PRIMATES
RECOMBINANT DNA
RNA
STRUCTURAL CHEMICAL ANALYSIS
TUMOR CELLS
VERTEBRATES