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Chromosome 11-linked determinant controls fetal globin expression and the fetal-to-adult globin switch

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
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Hybrids formed by fusing mouse erythroleukemia (MEL) cells with human fetal erythroid cells produce human fetal globin, but they switch to adult globin production as culture time advances. To obtain information on the chromosomal assignment of the elements that control ..gamma..-to-..beta.. switching, the authors analyzed the chromosomal composition of hybrids producing exclusively or predominantly human fetal globin and hybrids producing only adult human globin. No human chromosome was consistently present in hybrids expressing fetal globin and consistently absent in hybrids expressing adult globin. Subcloning experiments demonstrated identical chromosomal compositions in subclones displaying the fetal globin program and those that had switched to expression of the adult globin program. These data indicate that retention of only one human chromosome -- i.e., chromosome 11 -- is sufficient for expression of human fetal globin and the subsequent ..gamma..-to-..beta.. switch. The results suggest that the ..gamma..-to-..beta.. switch is controlled either cis to the ..beta..-globin locus of by a trans-acting mechanism, the genes of which reside on human chromosome 11.

Research Organization:
Univ. of Washington, Seattle (USA)
OSTI ID:
6879253
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:22; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADULTS
AGE GROUPS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGY
CHEMISTRY
CHROMOSOMES
CYTOCHEMISTRY
DAYS LIVING RADIOISOTOPES
FETUSES
GENETICS
GLOBIN
HETEROCHROMOSOMES
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MAMMALS
MESSENGER-RNA
MICE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RNA
RODENTS
VERTEBRATES