Murine erythroleukemia cell line GM979 contains factors that can activate silent chromosomal human. gamma. -globin genes

Journal Article · Thu Mar 14 23:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.6.2530· OSTI ID:5015722

Zitnik, G; Hines, P; Stamatoyannopoulos, G; Papayannopoulou, T ^[1]

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1. Univ. of Washington, Seattle (United States)

The authors introduced a normal chromosome 11 into GM979 murine erythroleukemia cells by fusing them with Epstein-Barr virus-transformed lymphocytes from a normal individual. In contrast to precious data obtained with other murine erythroleukemia cells, they detected activation of human chromosomal {gamma}-globin genes in GM979 cells. GM979, unlike previously used murine erythroleukemia cell lines, expresses murine embryonic globin in addition to adult globin. While all the hybrids expressed {gamma}- and {beta}-globin, they displayed a wide range of {gamma}-globin expression in relation to that of {beta}-globin. No correlation, however, was found in quantitative expression between murine embryonic globin and human {gamma}-globin in these hybrids, suggesting that the two globins are regulated independently, at least in this cell line. These data indicate that {gamma}-globin genes from normal, nonerythroid chromosomes are not irreversibly silenced, and they can be activated by a positive trans factor(s) present in GM979 cells.

• OSTI ID: 5015722
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:6; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

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550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL ACTIVATION
CHROMOSOMES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
GENE REGULATION
GENES
GLOBINS
HETEROCHROMOSOMES
IMMUNE SYSTEM DISEASES
ISOTOPES
LEUKEMIA
LEUKOCYTES
LIGHT NUCLEI
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MESSENGER-RNA
MICE
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
RODENTS
SOMATIC CELLS
TUMOR CELLS
VERTEBRATES