Interaction of protein with DNA in vitro
One of the important means to control eukaryotic gene expression involves the binding of proteins to specific sites in the promoter and other regulatory regions of the gene. This chapter is devoted to methodology for identifying DNA sequences that are bound in vitro by proteins in crude nuclear extracts. The authors present four methods of detection: nitrocellulose filter binding, mobility shift, exonuclease III protection, and DNAse I protection. In addition to providing assay conditions, they discuss some applications of the different methods. Very often data from protein-DNA interaction studies permit ambiguous interpretations. They there discuss control experiments designed to help the researcher to evaluate data.
- OSTI ID:
- 6879061
- Journal Information:
- Methods Enzymol.; (United States), Journal Name: Methods Enzymol.; (United States) Vol. 152; ISSN MENZA
- Country of Publication:
- United States
- Language:
- English
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62 RADIOLOGY AND NUCLEAR MEDICINE
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BIOCHEMISTRY
CARBOHYDRATES
CATIONS
CELLULOSE ESTERS
CHARGED PARTICLES
CHEMICAL EXPLOSIVES
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DAYS LIVING RADIOISOTOPES
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GENE REPRESSORS
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IN VITRO
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LABELLING
LIGHT NUCLEI
MAGNESIUM COMPOUNDS
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