Urinary excretion of unmetabolized benzene as an indicator of benzene exposure

Journal Article · Mon Mar 01 04:00:00 EST 1993 · Journal of Toxicology and Environmental Health; (United States)

DOI:https://doi.org/10.1080/15287399309531715· OSTI ID:6878108

Ghittori, S; Fiorentino, M L; Maestri, L; Cordioli, G; Imbriani, M ^[1]

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1. Medical Centre of Pavia (Italy)

Benzene concentrations in urine samples (Cu, ng/L) from 110 workers exposed to benzene in chemical plants and gasoline pumps were determined by injecting urine supernate into a gas chromatograph. The urine was saturated with anhydrous N2SO4 to facilitate the passage of benzene in the air over the urine. The solvent was stripped from the urine surface and concentrated on an adsorbent substrate (Carbotrap tube) by means of a suction pump (flow rate 150 ml/m). Wash-up of the head space was achieved by simultaneous intake of filtered air through charcoal. Benzene was thermically desorbed and injected in a column (thermal tube disorder, Supelco; 370 degrees C thermal flash; borosilicate capillary glass column SPB-1, 60 m length, 0.75 mm ID, 1 microns film thickness; GC Dani 8580-FID). Benzene concentrations in the urine from 40 non-exposed subjects (20 smokers > 20 cigarette/d and 20 nonsmokers) were also determined [median value of 790 ng/L (10.17 nmol/L) and 131 ng/L (1.70 nmol/L), respectively]. The 8-h time-weighted exposure intensity (Cl, micrograms/m3) of individual workers was monitored by means of charcoal tubes. The median value for exposure to benzene was 736 micrograms/m3 (9.42 mumol/m3) [geometric standard deviation (GSD) = 2.99; range 64 micrograms/m3 (0.82 mumol/m3) to 13,387 micrograms/m3 (171.30 mumol/m3)]. The following linear correlation was found between benzene concentrations in urine (Cu, ng/L) and benzene concentrations in the breathing zone (Cl, micrograms/m3): log(Cu) = 0.645 x log(Cl) + 1.399 r = .559, n = 110, p < .0001 With exclusion of workers who smoked from the study, the correlation between air benzene concentration and benzene measured in urine was: log(Cu) = 0.872 x log(Cl) + 0.6 r = .763, n = 63, p < .0001 The study results indicate that the urinary level of benzene is an indicator of occupational exposure to benzene.

• OSTI ID: 6878108
• Journal Information: Journal of Toxicology and Environmental Health; (United States), Journal Name: Journal of Toxicology and Environmental Health; (United States) Vol. 38:3; ISSN 0098-4108; ISSN JTEHD6
• Country of Publication: United States
• Language: English

Related Subjects

02 PETROLEUM
020600 -- Petroleum-- Health &amp; Safety
550500 -- Metabolism
560300* -- Chemicals Metabolism &amp; Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BENZENE
BIOLOGICAL ACCUMULATION
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CHEMICAL INDUSTRY
CHEMICAL PLANTS
CHROMATOGRAPHY
CLEARANCE
EXCRETION
FUELS
GAS CHROMATOGRAPHY
GASOLINE
HYDROCARBONS
INDUSTRIAL PLANTS
INDUSTRY
LIQUID FUELS
MATERIALS
MATHEMATICS
OCCUPATIONAL EXPOSURE
ORGANIC COMPOUNDS
PETROLEUM PRODUCTS
REGRESSION ANALYSIS
SEPARATION PROCESSES
STATISTICS
URINE
WASTES