Characterization of epidermal growth factor receptors on plasma membranes isolated from rat gastric mucosa

Journal Article · Fri Jun 01 00:00:00 EDT 1990 · Pharmaceutical Research; (USA)

DOI:https://doi.org/10.1023/A:1015838816061· OSTI ID:6875205

Hori, R; Nomura, H; Iwakawa, S; Okumura, K ^[1]

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1. Kyoto Univ. Hospital (Japan)

The binding of human epidermal growth factor (hEGF), beta-urogastrone, to plasma membranes isolated from rat gastric mucosa was studied to characterize gastric EGF receptors. The binding of ({sup 125}I)hEGF was temperature dependent, reversible, and saturable. A single class of binding sites for EGF with a dissociation constant of 0.42 nM and maximal binding capacity of 42 fmol/mg protein was suggested. There was little change in the binding of ({sup 125}I)hEGF upon addition of peptide hormones (secretin, insulin), antiulcer drugs (cimetidine), or an ulcer-inducing reagent (aspirin). Cross-linking of ({sup 125}I)hEGF to gastric plasma membranes with the use of disuccinimidyl suberate resulted in the labeling of a protein of 150 kDa. These results indicate the presence of EGF receptors on plasma membranes of rat gastric mucosa.

• OSTI ID: 6875205
• Journal Information: Pharmaceutical Research; (USA), Journal Name: Pharmaceutical Research; (USA) Vol. 7:6; ISSN 0724-8741; ISSN PHREE
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETYLSALICYLIC ACID
ANALGESICS
ANIMAL TISSUES
ANIMALS
ANTIPYRETICS
BACTERIA
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL HALF-LIFE
BODY
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHEMICAL COMPOSITION
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DOGS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EPIDERMIS
EPITHELIUM
ESCHERICHIA COLI
GASTROINTESTINAL TRACT
GROWTH FACTORS
HORMONES
HYDROXY ACIDS
IN VITRO
INSULIN
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MAN
MEMBRANE PROTEINS
MEMBRANES
MICROORGANISMS
MITOGENS
MUCOUS MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PRIMATES
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
REAGENTS
RECEPTORS
RODENTS
SECRETIN
SKIN
TEMPERATURE DEPENDENCE
TISSUES
TRACER TECHNIQUES
VERTEBRATES