DNA probe for lactobacillus delbrueckii

Journal Article · Fri Jun 01 00:00:00 EDT 1990 · Applied and Environmental Microbiology; (USA)

OSTI ID:6874740

Delley, M; Mollet, B; Hottinger, H ^[1]

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1. Nestle Research Centre, Lausanne (Switzerland)

From a genomic DNA library of Lactobacillus delbrueckii subsp. bulgaricus, a clone was isolated which complements a leucine auxotrophy of an Escherichia coli strain (GE891). Subsequent analysis of the clone indicated that it could serve as a specific DNA probe. Dot-blot hybridizations with over 40 different Lactobacillus strains showed that this clone specifically recognized L. delbrueckii subsp. delbrueckii, bulgaricus, and lactis. The sensitivity of the method was tested by using an {alpha}-{sup 32}P-labeled probe.

• OSTI ID: 6874740
• Journal Information: Applied and Environmental Microbiology; (USA), Vol. 56:6; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA
BIOASSAY
DNA HYBRIDIZATION
DNA-CLONING
ESCHERICHIA COLI
LACTOBACILLUS
PHOSPHORUS 32
TRACER TECHNIQUES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CLONING
DAYS LIVING RADIOISOTOPES
HYBRIDIZATION
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
550201* - Biochemistry- Tracer Techniques