Hydroxylated PAHs in bile of deep-sea fish. Relationship with xenobiotic metabolizing enzymes

Journal Article · Sun Aug 15 04:00:00 EDT 1999 · Environmental Science and Technology

DOI:https://doi.org/10.1021/es9902322· OSTI ID:687387

Escartin, E; Porte, C ^[1]

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1. IIQAB-CSIC, Barcelona (Spain). Environmental Chemistry Dept.

Polycyclic aromatic hydrocarbon (PAH) pollution in deep-sea environments has been assessed by measuring bile PAH metabolites in deep-sea fish. Five species from the NW Mediterranean were selected for the study: Coryphaenoides guentheri, Lepidion lepidion, Mora moro, Bathypterois mediterraneus, and Alepocephalus rostratus. Bile crude samples were directly analyzed by HPLC-fluorescence at the excitation/emission wavelengths of benzo[a]pyrene. Differences among sampling sites were recorded, which suggests that coastal discharges of contaminants may reach these remote areas. Subsequently, a number of bile samples were hydrolyzed and analyzed by gas chromatography--mass spectrometry (GC-MS) for the determination of individual PAHs. 1-Pyrenol and 2-phenylphenol were among the most abundant compounds detected. The results obtained confirm the long-range transport of PAHs to deep-sea environments, subsequent exposure of fish inhabiting those remote areas, and its ability to metabolize and excrete them through the bile. The data also describe hepatic enzymes (cytochrome P450 and glutathione S-transferases) that appear to be as catalytically efficient as those in shallow water species.

• OSTI ID: 687387
• Journal Information: Environmental Science and Technology, Journal Name: Environmental Science and Technology Journal Issue: 16 Vol. 33; ISSN 0013-936X; ISSN ESTHAG
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BIOLOGICAL ACCUMULATION
CHEMICAL ANALYSIS
FOOD CHAINS
HYDROXYLASES
LONG-RANGE TRANSPORT
MEDITERRANEAN SEA
METABOLISM
POLYCYCLIC AROMATIC HYDROCARBONS
TRANSFERASES
WATER POLLUTION