Significance of Cytochrome P450 System Responses and Levels of Bile Fluorescent Aromatic Compounds in Marine Wildlife Following Oil Spills

Journal Article · Fri Jul 01 04:00:00 EDT 2005 · Marine Pollution Bulletin

DOI:https://doi.org/10.1016/j.marpolbul.2005.04.036· OSTI ID:15020665

Lee, Richard F; Anderson, Jack W

The relationships among cytochrome P450 induction in marine wildlife species, levels of fluorescent aromatic compounds (FAC) in their bile, the chemical composition of the inducing compounds, the significance of the exposure pathway, and any resulting injury, as a consequence of exposure to crude oil following a spill, are reviewed. Fish collected after oil spills often show increases in cytochrome P450 system activity, cytochrome P4501A (CYP1A) and bile fluorescent aromatic compounds (FAC), that are correlated with exposure to polycyclic aromatic hydrocarbons (PAH) in the oil. There is also some evidence for increases in bile FAC and induction of cytochrome P450 in marine birds and mammals after oil spills. However, when observed, increases in these exposure indicators are transitory and generally decrease to background levels within one year after the exposure. Laboratory studies have shown induction of cytochrome P450 systems occurs after exposure of fish to crude oil in water, sediment or food. Most of the PAH found in crude oil (dominantly 2- and 3-ring PAH) are not strong inducers of cytochrome P450. Exposure to the 4-ring chrysenes or the photooxidized products of the PAH may account for the cytochrome P450 responses in fish collected from oil-spill sites. The contribution of non-spill background PAH, particularly combustion-derived (pyrogenic) PAH, to bile FAC and cytochrome P450 system responses can be confounding and needs to be considered when evaluating oil spill effects. The ubiquity of pyrogenic PAH makes it important to fully characterize all sources of PAH, including PAH from natural resources, e.g. retene, in oil spill studies. In addition, such parameters as species, sex, age, ambient temperature and season need to be taken into account. While increases in fish bile FAC and cytochrome P450 system responses, can together, be sensitive general indicators of PAH exposure after an oil spill, there is little unequivocal evidence to suggest a linkage to higher order biological effects, e.g. toxicity, lesions, reproductive failure.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 15020665
• Report Number(s): PNWD-SA-7005
• Journal Information: Marine Pollution Bulletin, Journal Name: Marine Pollution Bulletin Journal Issue: 7 Vol. 50
• Country of Publication: United States
• Language: English

Related Subjects

02 PETROLEUM
AMBIENT TEMPERATURE
AROMATICS
BILE
BIOLOGICAL EFFECTS
BIRDS
CHEMICAL COMPOSITION
CYTOCHROMES
FOOD
INDUCTION
MAMMALS
OIL SPILLS
PETROLEUM
POLYCYCLIC AROMATIC HYDROCARBONS
SEASONS
SEDIMENTS
TOXICITY
WATER
biomarker
oil
spill
cytochrome P450
bile
hydrocarbon
aromatic
CYP1A