( sup 14 C)urea and sup 58 Co-EDTA as reference indicators in hepatic multiple indicator dilution studies

Journal Article · Sun Jul 01 04:00:00 EDT 1990 · American Journal of Physiology; (USA)

OSTI ID:6873834

Pang, K S; Barker, F III; Schwab, A J; Goresky, C A ^[1]

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1. Univ. of Toronto, Ontario (Canada)

The space distribution and the processes underlying uptake of tracer substrate may be appraised by the multiple indicator dilution technique, after the simultaneous injection of noneliminated vascular ({sup 51}Cr-labeled red blood cells), extracellular ({sup 125}I-labeled albumin and ({sup 14}C)sucrose for high and low molecular weight interstitial space, respectively), and cellular ({sup 3}H{sub 2}O) indicators and tracer substrate. When tracer substrates and/or their metabolites containing {sup 14}C or {sup 3}H labels are being studied, it becomes necessary to find substitutions for the similarly labeled noneliminated indicators. In red blood cell-perfused rat livers, {sup 58}Co-EDTA is found to be a good replacement for the low molecular weight interstitial reference; it has a space distribution indistinguishable from that for ({sup 14}C)sucrose. ({sup 14}C)urea and {sup 3}H{sub 2}O, which distribute similarly in red blood cells, have similar outflow dilution profiles. With corrections for minor deviations in recovery (a ratio of 0.936 +/- 0.024 (SD)) and transit time (a ratio of 0.962 +/- 0.008 (SD)), the cellular water space can be closely approximated from the ({sup 14}C)-urea curve. 58Co-EDTA and ({sup 14}C)urea are reasonable substitutes for ({sup 14}C)sucrose and {sup 3}H{sub 2}O, respectively, in multiple indicator dilution liver perfusion studies for investigating transfer and removal characteristics of tracer substrates.

• OSTI ID: 6873834
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 259; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
AMIDES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACIDS
CHELATING AGENTS
CHROMIUM 51
CHROMIUM ISOTOPES
COBALT 58
COBALT ISOTOPES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISACCHARIDES
DISTRIBUTION
EDTA
ELECTRON CAPTURE RADIOISOTOPES
ERYTHROCYTES
EVEN-ODD NUCLEI
GLANDS
HOURS LIVING RADIOISOTOPES
HYDROGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE 125
IODINE ISOTOPES
ISOMERIC TRA
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
ISOTOPES
LABELLED COMPOUNDS
LIVER
MAMMALS
MATERIALS
METABOLISM
METABOLITES
MOLECULAR WEIGHT
NUCLEI
ODD-EVEN NUCLEI
OLIGOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PERFUSED ORGANS
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SACCHARIDES
SACCHAROSE
SUBSTRATES
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UREA
VERTEBRATES