Primary structure of a human protein which bears structural similarities to members of the rhodopsin/. beta. -adrenergic receptor family

Journal Article · Mon Sep 12 04:00:00 EDT 1988 · Nucleic Acids Research; (UK)

DOI:https://doi.org/10.1093/nar/16.17.8721· OSTI ID:6871786

Rapiejko, P J; George, S T; Malbon, C C ^[1]

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1. State Univ. of New York, Stony Brook (USA)

A human placental lambda gt11 cDNA library was screened with an antisera directed against a peptide of the first intracellular loop of the G-protein linked photoreceptor bovine rhodopsin. A clone, pHPS{sub 1}-2 was identified and a 1,788 bp EcoRI restriction fragment sequenced. The putative open reading frame encodes a protein that is similar to other membrane receptor proteins by a variety of criteria. Immunoblot analysis of a lambda lysogen of pHPS{sub 1}-2 indicates that the determinant of antibody recognition does not lie in the predicted open reading frame. This determinant arose as a fusion product of beta-galactosidase and the extreme 5{prime} region of pHPS{sub 1}-2. The nature of this putative protein is undetermined.

• OSTI ID: 6871786
• Journal Information: Nucleic Acids Research; (UK), Journal Name: Nucleic Acids Research; (UK) Vol. 16:17; ISSN 0305-1048; ISSN NARHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
CATTLE
DNA
DNA HYBRIDIZATION
DOMESTIC ANIMALS
FEMALES
FETAL MEMBRANES
HYBRIDIZATION
IMMUNE SERUMS
MAMMALS
MAN
MEMBRANE PROTEINS
MEMBRANES
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PIGMENTS
PLACENTA
PRIMATES
PROTEINS
RECEPTORS
RECOMBINANT DNA
RHODOPSIN
RUMINANTS
VERTEBRATES
WOMEN