Characterization and chromosomal localization of the gene for human rhodopsin kinase
- Massachusetts Eye and Ear Infirmary, Boston, MA (United States); and others
G-protein-dependent receptor kinases (GRKs) play a key role in the adapatation of receptors to persistent stimuli. In rod photoreceptors rhodopsin kinase (RK) mediates rapid densensitization of rod photoreceptors to light by catalyzing phosphorylation of the visual pigment rhodopsin. To study the structure and mechanism of FRKs in human photoreceptors, we have isolated and characterized cDNA and genomic clones derived from the human RK locus using a bovine rhodopsin kinase cDNA fragment as a probe. The RK locus, assigned to chromosome 13 band q34, is composed of seven exons that encode a protein 92% identical in amino acid sequence to bovine rhodopsin kinase. The marked difference between the structure of this gene and that of another recently clone human GRK gene suggests the existence of a wide evolutionary gap between members of the GRK gene family. 39 refs., 3 figs.
- OSTI ID:
- 465994
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 35; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
Similar Records
Characterization and mapping of the human rhodopsin kinase gene and screening of the gene for mutations in patients with retinitis pigmentosa
Study of the orientation of retinal in bovine rhodopsin: the use of a photoactivatable retinal analog
Structures of Rhodopsin Kinase in Different Ligand States Reveal Key Elements Involved in G Protein-coupled Receptor Kinase Activation
Journal Article
·
Thu Sep 01 00:00:00 EDT 1994
· American Journal of Human Genetics
·
OSTI ID:133847
Study of the orientation of retinal in bovine rhodopsin: the use of a photoactivatable retinal analog
Conference
·
Fri May 01 00:00:00 EDT 1987
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:6180117
Structures of Rhodopsin Kinase in Different Ligand States Reveal Key Elements Involved in G Protein-coupled Receptor Kinase Activation
Journal Article
·
Wed Oct 08 00:00:00 EDT 2008
· J. Biol. Chem.
·
OSTI ID:1006672
Related Subjects
55 BIOLOGY AND MEDICINE
BASIC STUDIES
AMINO ACID SEQUENCE
BIOLOGICAL EVOLUTION
CATTLE
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
GENE MUTATIONS
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOME 13
MAN
NUCLEOTIDES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
POLYMERASE CHAIN REACTION
PROBES
RHODOPSIN
SENSE ORGANS DISEASES
STRUCTURE-ACTIVITY RELATIONSHIPS
BASIC STUDIES
AMINO ACID SEQUENCE
BIOLOGICAL EVOLUTION
CATTLE
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
GENE MUTATIONS
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOME 13
MAN
NUCLEOTIDES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
POLYMERASE CHAIN REACTION
PROBES
RHODOPSIN
SENSE ORGANS DISEASES
STRUCTURE-ACTIVITY RELATIONSHIPS