Interleukin 3 perfusion in W/Wv mice allows the development of macroscopic hematopoietic spleen colonies and restores cutaneous mast cell number

Journal Article · Sun Jul 01 00:00:00 EDT 1990 · Journal of Experimental Medicine; (USA)

DOI:https://doi.org/10.1084/jem.172.1.403· OSTI ID:6868948

Ody, C; Kindler, V; Vassalli, P ^[1]

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1. Univ. of Geneva (Switzerland)

The genetically anemic W/Wv mice are characterized by the inability of their bone marrow cells to form macroscopic pluripotent hematopoietic colonies in the spleen of irradiated recipients upon transfer (colony-forming units). Furthermore, they almost totally lack mast cells, notably in the skin. In the present study, we have tested the effect of recombinant murine interleukin 3 (rmIL-3) on W/Wv mice hematopoiesis. Transfer of W/Wv bone marrow cells into lethally irradiated recipients perfused with rmIL-3 is followed by the appearance of macroscopic spleen colonies. Moreover, perfusion of rmIL-3 in W/Wv mice: (a) restores almost normal total numbers of hematopoietic precursors (colony-forming cells), but without modification of anemia; and (b) leads to the appearance of a normal number of mastocytes in the skin.

• OSTI ID: 6868948
• Journal Information: Journal of Experimental Medicine; (USA), Journal Name: Journal of Experimental Medicine; (USA) Vol. 172:1; ISSN 0022-1007; ISSN JEMEA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANEMIAS
ANIMAL CELLS
ANIMALS
BIOLOGICAL FUNCTIONS
BODY
BONE MARROW CELLS
CELL CULTURES
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DISEASES
FUNCTIONS
GROWTH FACTORS
HEMATOPOIETIC SYSTEM
HEMIC DISEASES
IRRADIATION
LETHAL IRRADIATION
LYMPHOKINES
MAMMALS
MAST CELLS
MICE
MITOGENS
ORGANIC COMPOUNDS
ORGANS
PRECURSOR
PROTEINS
RODENTS
SOMATIC CELLS
SPLEEN
SYMPTOMS
TRANSPLANTS
VERTEBRATES