A method to enrich mouse hematopoietic stem cells
Hematopoietic stem cells form colonies in the spleens of lethally irradiated mice. The number of colonies is indicative of the number of stem cells present in the inoculum. In this article, a technique is presented for effecting a seven-fold enrichment of spleen colony-forming cells. Bone marrow cells are first segregated into ''red'' and ''white'' cell populations by centrifugation on Ficoll-Paque. Centrifugation of the ''white'' cell fraction in 75% Percoll concentrates the colony-forming cells in the top one-third of the gradient. The ability of these cells to repopulate and to cure the anemia of WBB6F1-W/Wv mice indicates that long-term functional pluripotent stem cells have not been destroyed or lost during the fractionation procedures. The segregation procedures enrich the colony-forming cell population from thalassemic as well as from normal mice.
- Research Organization:
- Jackson Laboratory, Bar Harbor, ME
- OSTI ID:
- 5577945
- Journal Information:
- Blood; (United States), Journal Name: Blood; (United States) Vol. 62:4; ISSN BLOOA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANEMIAS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BONE MARROW CELLS
CENTRIFUGATION
CHIMERAS
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DISEASES
HEMATOPOIETIC SYSTEM
HEMIC DISEASES
IRRADIATION
LETHAL IRRADIATION
MAMMALS
MICE
MOSAICISM
ORGANS
RADIATION EFFECTS
RODENTS
SEPARATION PROCESSES
SOMATIC CELLS
SPLEEN
SPLEEN COLONY FORMATION
STEM CELLS
SYMPTOMS
THALASSEMIA
ULTRACENTRIFUGATION
VERTEBRATES