Growth of ovine granulocyte-macrophage precursors in vitro without exogenous colony-stimulating activity

Journal Article · Tue Nov 01 04:00:00 EST 1983 · Am. J. Vet. Res.; (United States)

OSTI ID:6868885

Chandra, P; Joel, D D; Chanana, A D

Ovine granulocyte-macrophage colony-forming units (CFU-GM) from peripheral blood and bone marrow were cultured in vitro. The colony-stimulating activity (CSA) was provided by various conditioned-media previously reported to contain CSA and by homologous sheep serum (SS). The maximum number of CFU-GM was observed in the cultures containing SS without the addition of exogenous CSA. The CFU-GM appeared earlier in the cultures containing bone marrow cells when compared to the peripheral blood CFU-GM. Replacement of SS by bovine fetal serum resulted in suboptimal growth of ovine CFU-GM.

• Research Organization: Brookhaven National Lab., Upton, NY
• DOE Contract Number: AC02-76CH00016
• OSTI ID: 6868885
• Journal Information: Am. J. Vet. Res.; (United States), Journal Name: Am. J. Vet. Res.; (United States) Vol. 44:11; ISSN AJVRA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD SERUM
BODY
BODY FLUIDS
BONE MARROW
CELL CULTURES
CFU
CLONING
CONNECTIVE TISSUE CELLS
DOMESTIC ANIMALS
GROWTH
HEMATOPOIETIC SYSTEM
IN VITRO
LEUKOCYTES
MACROPHAGES
MAMMALS
MATERIALS
ORGANS
PHAGOCYTES
PRECURSOR
RUMINANTS
SHEEP
SOMATIC CELLS
TISSUES
VERTEBRATES