Radioprotection of hematopoietic tissues in mice by indomethacin

Journal Article · Tue May 01 04:00:00 EDT 1990 · Radiation Research; (USA)

DOI:https://doi.org/10.2307/3577605· OSTI ID:6867869

Nishiguchi, I; Furuta, Y; Hunter, N; Murray, D; Milas, L ^[1]

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1. Univ. of Texas M.D. Anderson Cancer Center, Houston (USA)

We recently reported that indomethacin, an inhibitor of prostaglandin (PG) synthesis, increased the radioresponse of PG-producing murine tumors, but it protected the hematopoietic system from radiation damage. Here we have investigated possible mechanisms responsible for the radioprotective effect of indomethacin. In the exogenous spleen colony assay, bone marrow cells from indomethacin-treated mice showed a similar radioresponse to those from mice not treated with indomethacin, thus excluding true radioprotection as a mechanism. Also, neither the total number of bone marrow cells nor the number of stem cells in bone marrow were affected by the treatment with indomethacin. However, indomethacin induced significant splenomegaly, which was associated with an increased number of both nucleated cells and hematopoietic stem cells in the spleen. The latter was determined by the exogenous spleen colony assay. Thus indomethacin protected hematopoietic tissue indirectly through stimulation of hematopoietic cells in the spleen. When indomethacin was combined with WR-2721, which is a true radioprotector, we obtained a greater radioprotective effect than with either used alone according to the endogenous spleen colony assay.

• OSTI ID: 6867869
• Journal Information: Radiation Research; (USA), Journal Name: Radiation Research; (USA) Vol. 122:2; ISSN 0033-7587; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BODY
BONE MARROW CELLS
COLONY FORMATION
CONNECTIVE TISSUE CELLS
ENZYME INHIBITORS
HEMATOPOIETIC SYSTEM
MAMMALS
MICE
ORGANS
RADIATION PROTECTION
RADIOSENSITIVITY EFFECTS
RODENTS
SOMATIC CELLS
SPLEEN
VERTEBRATES