Enzyme immobilization on tritylagarose

Journal Article · Mon Feb 01 04:00:00 EST 1982 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260240212· OSTI ID:6867474

Cashion, P; Javed, A; Harrison, D; Seeley, J; Lentini, V; Sathe, G

A method is described for the immobilization on tritylated agarose or Sepharose columns of a wide spectrum of enzymes, including types useful in contemporary biochemistry/molecular biology, many of which have never before been reported as immobilized. The method involves the formation of noncovalent hydrophobic bonds between the enzymes and trityl groups which are attached to the agarose by means of ether bonds. The immobilization of calf intestinal and E. coli alkaline phosphatases to tritylagarose is reported in detail. Their binding strength, binding capacity, and long-term stability (greater than six months) are described as a function of the salt concentration, pH, buffer type, and degree of agarose substitution. Homologies are noted between tritylagarose-bound and membrane-bound phosphatases. This method compares favorably with other methods, covalent or otherwise, reported to date, in terms of the enzyme immobilization yield (ca. 100%), the mildness of conditions, resulting, in most cases, in the retention of a high degree of activity, the ease and speed of the manipulations, and the long-term stability of the immobilized enzyme. Further, it is noted that highly tritylated and crosslinked Sephadex G10 selectively and mildly removes detergents from enzyme solutions. (Refs. 64).

• Research Organization: Dept of Biology, Univ of New Brunswick, Fredericton, New Brunswick E3B 6E1, Canada
• OSTI ID: 6867474
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 24:2; ISSN BIBIA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALKALINE PHOSPHATASE
BIOCHEMISTRY
CHEMICAL PREPARATION
CHEMISTRY
COLLOIDS
DISPERSIONS
ENZYME ACTIVITY
ENZYMES
ESTERASES
HYDROLASES
IMMOBILIZED ENZYMES
PHOSPHATASES
STABILITY
SYNTHESIS