Exceptional responsiveness of certain human myeloid leukemia cells to colony-stimulating activity

Journal Article · Mon Jan 01 04:00:00 EST 1979 · Blood; (United States)

OSTI ID:6864873

Brennan, J K; DiPersio, J F; Abboud, C N; Lichtman, M A

We have studied the marrow cells from a patient with acute myeloid leukemia (AML) for their responsiveness to colony-stimulating activity (CSA) in vitro. The AML cells were stimulated by CSA to rapid and extended growth in liquid culture. In the absence of CSA, the majority of cells died. CSA also stimulated the clonal growth of AML cells, and the minimum requirement for CSA was one-tenth to one-fiftieth that required to stimulate the growth of normal marrow CFU-C. CSA for AML cells was eluted from Sephacryl S-200 columns in fractions that represented an apparent molecular weight of 45,000 daltons. During remission, the patient's marrow cells did not grow in liquid culture and produced normal numbers of granulocytic and erythroid colonies in responsive to CSA and erythropoietin. These studies indicate that certain AML cells are extraordinarily responsive to CSA, an in vitro mediator of normal granulopoiesis.

• Research Organization: Univ. of Rochester School of Medicine and Dentistry, NY
• OSTI ID: 6864873
• Journal Information: Blood; (United States), Journal Name: Blood; (United States) Vol. 54; ISSN BLOOA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
CFU
COLONY FORMATION
CULTURE MEDIA
DISEASES
DOSE-RESPONSE RELATIONSHIPS
HEMIC DISEASES
LEUKEMIA
MYELOID LEUKEMIA
NEOPLASMS
STIMULATION
TUMOR CELLS