Incorporation of ( sup 14 C)-palmitate into lipids of Brassica cells during the induction of freezing tolerance

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6864610

Lynch, D V; Joseph, R A ^[1]

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1. Cornell Univ., Ithaca, NY (USA)

Changes in plasma membrane lipid composition have been causally related to increased freezing tolerance. Studies of lipid metabolism during ABA induction of freezing tolerance in Brassica napus suspension cultures were undertaken. Cells were labeled with ({sup 14}C)-palmitate four days after transfer to fresh medium (control) or medium containing ABA (which increases freezing tolerance). At times between one and 20 hrs after labeling, ABA-treated cells incorporated almost twice the amount of label as controls cells. Approximately 80% of the radioactivity was associated with neutral lipids in ABA-treated cells and controls. Incorporation of label into total cellular polar lipids was 4.9 {times} 10{sup 5} dpm/mg protein for control cells and 1 {times} 10{sup 6} dpm/mg protein for cells transferred to medium containing ABA. Analysis of lipids following alkaline hydrolysis indicated that incorporation of ({sup 14}C)-palmitate into glucosylceramide of ABA-treated cells was less than 60% of control values when expressed relative to that of the total polar lipids. Incorporation into ceramides was also depressed in ABA-treated cells.

• OSTI ID: 6864610
• Journal Information: Plant Physiology, Supplement; (USA), Journal Name: Plant Physiology, Supplement; (USA) Vol. 89:4; ISSN PPYSA; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560200* -- Thermal Effects
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE HYDROLYSIS
BIOLOGICAL ADAPTATION
BRASSICA
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DECOMPOSITION
FOOD
FREEZING
HEXADECANOIC ACID
HYDROLYSIS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
LYSIS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
METABOLISM
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANT CELLS
PLANTS
SOLVOLYSIS
TEMPERATURE EFFECTS
TRACER TECHNIQUES
VEGETABLES