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Effects of tetracaine on insulin release and calcium handling by rat pancreatic islets

Journal Article · · Am. J. Physiol.; (United States)
OSTI ID:6864428
; ;

The effects of tetracaine on insulin release and /sup 45/Ca/sup 2 +/ handling by rat pancreatic islets have been studied under basal, glucose-stimulated, and 3-isobutyl-1-methylxanthine (IBMX)-stimulated conditions. Islets were isolated by the use of collagenase and used either directly (freshly isolated islets) or after a period under tissue culture conditions. Tetracaine was found to stimulate insulin release under basal conditions, to inhibit glucose-stimulated insulin release, and to potentiate insulin release stimulated by IBMX. In studies on the mechanisms underlying these effects, tetracaine was found to decrease glucose-stimulated net retention of /sup 45/Ca/sup 2 +/ (by an action to block the voltage-dependent Ca channels) and to mobilize Ca/sup 2 +/ from intracellular stores. These two actions form the basis for the inhibition of glucose-stimulated insulin release, which depends heavily on Ca/sup 2 +/ entry via the voltage-dependent channels and the synergism with IBMX to potentiate release. No inhibition of IBMX-stimulated release occurs because IBMX does not use the voltage-dependent channels to raise intracellular Ca/sup 2 +/.

Research Organization:
Cornell Univ., Ithaca, NY (USA)
OSTI ID:
6864428
Journal Information:
Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 252:6; ISSN AJPHA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ALKALINE EARTH ISOTOPES
ANESTHETICS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CALCIUM 45
CALCIUM ISOTOPES
CARBOHYDRATES
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DRUGS
ENDOCRINE GLANDS
EVEN-ODD NUCLEI
GLANDS
GLUCOSE
HEXOSES
HORMONES
INSULIN
INTERMEDIATE MASS NUCLEI
ISOTOPES
MAMMALS
MONOSACCHARIDES
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PEPTIDE HORMONES
PERFUSED TISSUES
PHYSIOLOGY
RADIOISOTOPES
RATS
RODENTS
SACCHARIDES
SECRETION
TISSUES
UPTAKE
VERTEBRATES