Clonidine promotes the accumulation of /sup 45/Ca in pancreatic beta-cell organelles

Journal Article · Wed Nov 30 23:00:00 EST 1983 · Res. Commun. Chem. Pathol. Pharmacol.; (United States)

OSTI ID:6742940

Andersson, T; Nygren, P

Glucose-stimulated insulin release from pancreatic islets of ob/ob-mice was inhibited by 10(-9) M of the alpha 2-adrenergic agonist clonidine. This inhibitory effect was abolished by 10(-7) M of the antagonist yohimbine. Loading the islets with /sup 45/Ca during the clonidine exposure followed by isolation of subcellular fractions under conditions known to minimize the /sup 45/Ca redistribution resulted in increased accumulation of the isotope in the mitochondrial and microsomal fractions. It is suggested that clonidine inhibits glucose-stimulated insulin release by increasing the organelle sequestration of Ca2+.

• Research Organization: Univ. of Uppsala, Sweden
• OSTI ID: 6742940
• Journal Information: Res. Commun. Chem. Pathol. Pharmacol.; (United States), Journal Name: Res. Commun. Chem. Pathol. Pharmacol.; (United States) Vol. 42:3; ISSN RCOCB
• Country of Publication: United States
• Language: English

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560172* -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Animals-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADRENAL HORMONES
ADRENALINE
ALKALINE EARTH ISOTOPES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CALCIUM 45
CALCIUM ISOTOPES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DRUGS
ENDOCRINE GLANDS
EVEN-ODD NUCLEI
GLANDS
HORMONES
INHIBITION
INSULIN
INTERMEDIATE MASS NUCLEI
ISOTOPES
MAMMALS
MICE
MICROSOMES
MITOCHONDRIA
NEUROREGULATORS
NUCLEI
ORGANOIDS
ORGANS
PANCREAS
PEPTIDE HORMONES
RADIOISOTOPES
RODENTS
STEROID HORMONES
STIMULATION
SYMPATHOMIMETICS
UPTAKE
VERTEBRATES