Tissue distribution and vitamin D dependence of IMCAL in the rat

Journal Article · Tue Sep 01 04:00:00 EDT 1987 · Am. J. Physiol.; (United States)

OSTI ID:6863507

Kowarski, S; Cowen, L A; Takahashi, M T; Schachter, D

Integral membrane calcium-binding protein (IMCAL) is a vitamin D-dependent integral membrane protein that binds calcium with relatively high affinity. Specific immunoassays for IMCAL utilizing rabbit polyclonal and mouse monoclonal antibodies were developed and applied to studies of its tissue distribution and regulation by vitamin D/sub 3/ and dietary calcium in the rat. The results indicate that vitamin D-dependent, cross-reactive protein is present in small intestinal mucosa, cecal mucosa, bone, kidney, brain, testis, heart, lung, spleen, and skin. Rats maintained on a low- (0.02%) compared with a high- (2.0%) calcium diet had significantly higher content of IMCAL in duodenal mucosa, cecal mucosa, bone, kidney, brain, testis, and heart. Treatment of rats on the high-calcium diet with 1,25-dihydroxyvitamin D/sub 3/ increased the IMCAL content of the duodenal mucosa, cecal mucosa, and kidney. The widespread tissue distribution of vitamin D-dependent IMCAL, its close correlation in intestinal mucosa with the calcium transport mechanism, and its occurrence in isolated preparations of enterocyte plasma membranes (microvillus and basolateral membranes) suggest that the protein is involved in the regulation of calcium flux in a number of cell types.

• Research Organization: Columbia Univ. College of Physicians and Surgeons, New York, NY (USA)
• OSTI ID: 6863507
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 253:3; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALINE EARTH METAL COMPOUNDS
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BODY
BRAIN
CALCIUM COMPOUNDS
CARDIOVASCULAR SYSTEM
CENTRAL NERVOUS SYSTEM
CHEMISTRY
CHOLECALCIFEROL
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
GASTROINTESTINAL TRACT
GONADS
HEART
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
INTESTINES
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LUNGS
MALE GENITALS
MEMBRANE TRANSPORT
MEMBRANES
MUCOUS MEMBRANES
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RESPIRATORY SYSTEM
SKIN
SMALL INTESTINE
SPLEEN
TESTES
TISSUE DISTRIBUTION
TRACER TECHNIQUES
VITAMIN D
VITAMINS