Genetic and molecular characterization of genomic regions surrounding specific loci of the mouse
Mutations detected by the mouse specific-locus test (SLT) include multilocus deletions as well as intragenic lesions. Genetic analyses have characterized sets of presumed overlapping deletions and have mapped previously unrecognized genes to the regions surrounding each of several specific loci. Molecular entry to one of these regions, d se, was achieved by utilizing a viral integration at, or near, a marker locus. Presumed deletions were shown to be, in fact deleted for DNA sequences, and the physical map was oriented relative to the earlier functional map. Presently, a random-clone approach is being used for initiating molecular characterization of regions, which, in aggregate, span a minimum of 9 cM. Mapping to subregions already identified by functional units will facilitate the generation of comprehensive molecular maps and the identification of numerous structure-function correlations for the regions. Results of the genetic and molecular analyses of multilocus deletions have enhanced the value of the SLT by adding qualitative to quantitative capabilities. Studies of the heterozygous effects of deletions (which are the predominant lesions induced by many mutagens) provide information important to assessment of genetic risk. Long deletions are, further, providing tools for targeted mutagenesis studies that will generate information on the number of loci within segments of defined length that are capable of mutating to detectable alleles, as well as providing new mutations important for strategies of refining molecular and functional maps. 28 refs., 2 tabs.
- Research Organization:
- Oak Ridge National Lab., TN (USA)
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 6857655
- Report Number(s):
- CONF-8703112-2; ON: DE87009020
- Resource Relation:
- Conference: Banbury Centre conference on mammalian cell mutagenesis, Cold Springs Harbor, NY, USA, 22 Mar 1987
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
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GENETIC MAPPING
CELL CULTURES
HETEROCHROMOSOMES
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MUTATIONS
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