Factors that affect the molecular nature of germ-line mutations recovered in the mouse specific-locus test
- Oak Ridge National Lab., TN (United States)
The morphological specific locus test (SLT), which allows the scoring of 2,000 loci/hr/person, has been in use for four decades for measuring mammalian germ-line mutation rates under various conditions of exposure. More recently, the SLT's capabilities for the qualitative characterization of mutations have been exploited. The large sets of mutations centered on specific loci that have been accumulated over the years, including sets of nested deletions, have provided prime material for fine-structure genetic analyses. Subsequent molecular entry to these regions has led to intensive physical/functional mapping of megabase segments of the genome. In turn, these investigations have generated genetic and molecular tools for analyzing individual mutations as to extent and nature of the genomic lesion. These and related quantitative findings now make it possible to optimize conditions for the use of mutagens in providing desired types of mutations as tools.
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 5560615
- Journal Information:
- Environmental and Molecular Mutagenesis; (United States), Vol. 18:4; ISSN 0893-6692
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GENE MUTATIONS
MOLECULAR BIOLOGY
CHLORAMBUCIL
CHROMOSOMAL ABERRATIONS
GENETIC MAPPING
GERM CELLS
MUTAGENESIS
MUTATION FREQUENCY
NITROSO COMPOUNDS
SPERMATOZOA
STEM CELLS
AMINES
ANIMAL CELLS
ANTINEOPLASTIC DRUGS
CARBOXYLIC ACIDS
DRUGS
GAMETES
MAPPING
MONOCARBOXYLIC ACIDS
MUTATIONS
ORGANIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
SOMATIC CELLS
550200* - Biochemistry